验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16016-1-AP targets TXNDC4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | TXNDC4 fusion protein Ag8818 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | endoplasmic reticulum protein 44 |
| Synonyms | ERp44, ER protein 44, UNQ532/PRO1075, Thioredoxin domain-containing protein 4, PDIA10 |
| Calculated Molecular Weight | 406 aa, 47 kDa |
| Observed Molecular Weight | 44-47 kDa |
| GenBank Accession Number | BC005374 |
| Gene Symbol | ERP44 |
| Gene ID (NCBI) | 23071 |
| RRID | AB_1640025 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BS26 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| IHC protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| IF protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| IP protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. | ||
Antioxid Redox Signal Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM). | ||
J Transl Med ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.
| ||
Traffic Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells. | ||
BMC Cancer Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma | ||
Tissue Cell Endoplasmic reticulum stress of endometrial mesenchymal stem cells in endometriosis |
