验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16016-1-AP targets TXNDC4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | TXNDC4 fusion protein Ag8818 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endoplasmic reticulum protein 44 |
| 别名 | ERp44, ER protein 44, UNQ532/PRO1075, Thioredoxin domain-containing protein 4, PDIA10 |
| 计算分子量 | 406 aa, 47 kDa |
| 观测分子量 | 44-47 kDa |
| GenBank蛋白编号 | BC005374 |
| 基因名称 | ERP44 |
| Gene ID (NCBI) | 23071 |
| RRID | AB_1640025 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BS26 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| IHC protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| IF protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| IP protocol for TXNDC4 antibody 16016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. | ||
Antioxid Redox Signal Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM). | ||
J Transl Med ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.
| ||
Traffic Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells. | ||
BMC Cancer Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma | ||
Tissue Cell Endoplasmic reticulum stress of endometrial mesenchymal stem cells in endometriosis |
