验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11630-1-AP targets MIG6; ERRFI1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MIG6; ERRFI1 fusion protein Ag2202 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ERBB receptor feedback inhibitor 1 |
| 别名 | ERRFI1, Mitogen-inducible gene 6 protein, MIG-6, MIG6, MIG 6 |
| 计算分子量 | 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC025337 |
| 基因名称 | MIG6 |
| Gene ID (NCBI) | 54206 |
| RRID | AB_2101525 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UJM3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| IHC protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| IF protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng Profiling of protein-protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors. | ||
Proc Natl Acad Sci U S A Cartilage-specific deletion of Mig-6 results in osteoarthritis-like disorder with excessive articular chondrocyte proliferation. | ||
Nucleic Acids Res A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells. | ||
Aging Cell METTL14 affects UVB-induced human dermal fibroblasts photoaging via miR-100-3p biogenesis in an m6 A-dependent manner | ||
EMBO Rep MIG-6 is essential for promoting glucose metabolic reprogramming and tumor growth in triple-negative breast cancer. | ||
Arch Pharm Res Selumetinib overcomes gefitinib primary and acquired resistance by regulating MIG6/STAT3 in NSCLC
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