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ESD Polyclonal antibody

ESD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15244-1-AP

Print datasheet

Synonyms

S-formylglutathione hydrolase, Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12

验证数据展示

  • WB analysis of K-562 using 15244-1-AP

    WB analysis of K-562 using 15244-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15244-1-AP

    WB analysis of HeLa using 15244-1-AP

    WB result of ESD antibody (15244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ESD transfected HeLa cells.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15244-1-AP targets ESD in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ESD fusion protein Ag7457 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name esterase D/formylglutathione hydrolase
Synonyms S-formylglutathione hydrolase, Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC001169
Gene Symbol ESD
Gene ID (NCBI) 2098
RRIDAB_2101686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

实验方案

Product Specific Protocols
WB protocol for ESD antibody 15244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols