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ESD Monoclonal antibody

ESD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G1B5

Cat no : 67069-3-Ig

Print datasheet

Synonyms

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

验证数据展示

  • WB analysis using 67069-3-Ig

    WB analysis using 67069-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-3-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHCT 116 cells, Caco-2 cells, HepG2 cells, Jurkat cells, K-562 cells, Ramos cells, pig brain tissue, rat brain tissue, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67069-3-Ig targets ESD in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ESD fusion protein Ag7487 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name esterase D/formylglutathione hydrolase
Synonyms Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56
Calculated Molecular Weight 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC001169
Gene Symbol ESD
Gene ID (NCBI) 2098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

实验方案

Product Specific Protocols
WB protocol for ESD antibody 67069-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols