• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ESF1 Polyclonal antibody

ESF1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 23496-1-AP

Print datasheet

Synonyms

ABT1 associated protein, ABTAP, C20orf6, ESF1, ESF1 homolog, HDCMC28P

验证数据展示

  • WB analysis of mouse heart using 23496-1-AP

    WB analysis of mouse heart using 23496-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 23496-1-AP (ESF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 23496-1-AP

    IF Staining of MCF-7 using 23496-1-AP

    Immunofluorescent analysis of MCF-7 cells using 23496-1-AP (ESF1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23496-1-AP is ESF1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ESF1 fusion protein Ag19037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ESF1, nucleolar pre-rRNA processing protein, homolog (S. cerevisiae)
Synonyms ABT1 associated protein, ABTAP, C20orf6, ESF1, ESF1 homolog, HDCMC28P
Calculated Molecular Weight 851 aa, 99 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC146542
Gene Symbol ESF1
Gene ID (NCBI) 51575
RRIDAB_2879287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H501
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ESF1 antibody 23496-1-APDownload protocol
IF protocol for ESF1 antibody 23496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Clin Proteomics

ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis

Authors - Qing Yu
View Article
humanIF

bioRxiv

Mapping and engineering RNA-controlled architecture of the multiphase nucleolus

Authors - S A Quinodoz
View Article