验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32079-1-AP targets ESM1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ESM1 fusion protein Ag36144 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endothelial cell-specific molecule 1 |
| 别名 | |
| 计算分子量 | 184 aa, 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC011989 |
| 基因名称 | ESM1 |
| Gene ID (NCBI) | 11082 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NQ30 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ESM1, also known as endothelial cell-specific molecule 1 or endocan, is a protein-coding gene that encodes a secreted protein. It is primarily expressed in endothelial cells of human lung and kidney tissues, and its expression is regulated by cytokines, suggesting a role in endothelium-dependent pathological disorders. ESM1 has been implicated in various diseases, including gastric cancer, hypertension, and cervical cancer. In cervical cancer, ESM1 is overexpressed and acts as an independent prognostic factor associated with poor clinical outcomes. It promotes carcinoma angiogenesis and cervical squamous cell carcinoma progression through the VEGF/ERK signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ESM1 antibody 32079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |