- Featured Product
- KD/KO Validated
ER Polyclonal antibody
ER Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
127
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue, MCF-7 cells |
Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21244-1-AP targets ER in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine, goat |
Immunogen | ER fusion protein Ag15738 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | estrogen receptor 1 |
Synonyms | ESR1, ESR, ERα, ER-alpha, Era |
Calculated Molecular Weight | 595 aa, 66 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC128573 |
Gene Symbol | ER |
Gene ID (NCBI) | 2099 |
RRID | AB_11042600 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P03372 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ER antibody 21244-1-AP | Download protocol |
IHC protocol for ER antibody 21244-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII | ||
J Biomed Sci Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines. | ||
Theranostics Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype | ||
J Hazard Mater Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice. | ||
J Med Chem Discovery of Thiochroman Derivatives as Potent, Oral Selective Estrogen Receptor Degraders and Antagonists for the Treatment of Endocrine-Resistant Breast Cancer | ||
J Environ Sci (China) The effects of 17β-trenbolone and bisphenol A on sexual behavior and social dominance via the hypothalamic-pituitary-gonadal axis in male mice |