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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 30694-1-AP

    WB analysis using 30694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30694-1-AP (ESR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

ESR2 Polyclonal antibody

ESR2 Polyclonal Antibody for WB, ELISA
Cat No. 30694-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, PC-3 cells, SH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30694-1-AP targets ESR2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ESR2 fusion protein Ag33524 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name estrogen receptor 2 (ER beta)
Synonyms ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2
Calculated Molecular Weight 59 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC024181
Gene Symbol ESR2
Gene ID (NCBI) 2100
RRIDAB_3086390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92731
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa.

实验方案

Product Specific Protocols
WB protocol for ESR2 antibody 30694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols