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验证数据展示

  • WB analysis of mouse brain using 14657-1-AP

    WB analysis of mouse brain using 14657-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14657-1-AP (ESX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14657-1-AP

    IP experiment of mouse brain using 14657-1-AP

    IP result of anti-ESX1 (IP:14657-1-AP, 4ug; Detection:14657-1-AP 1:500) with mouse brain tissue lysate 3440ug.

ESX1 Polyclonal antibody

ESX1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 14657-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

ESX homeobox 1, ESX1, ESX1L, ESX1R, ESXR1, Homeobox protein ESX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14657-1-AP targets ESX1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 ESX1 fusion protein Ag6269 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ESX homeobox 1
别名 ESX homeobox 1, ESX1, ESX1L, ESX1R, ESXR1, Homeobox protein ESX1
计算分子量 44 kDa
观测分子量 65 kDa
GenBank蛋白编号BC053599
基因名称 ESX1
Gene ID (NCBI) 80712
RRIDAB_10694138
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N693
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ESX1, also named as ESX1L and ESX1R , is an X-linked homeobox gene primarily expressed in the placenta and testis, with physiological functions in placenta/fetus development and spermatogenesis. It has a substantively different C-terminal region subject to strong purifying selection. ESX1 can be cleaved into 2 part: ESXR1-N(45kd) and ESXR1-C(20kd). ESXR1-N acts as a transcriptional repressor. ESXR1-C has the ability to inhibit cyclin turnover.

实验方案

Product Specific Protocols
WB protocol for ESX1 antibody 14657-1-APDownload protocol
IP protocol for ESX1 antibody 14657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols