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ESX1 Polyclonal antibody

ESX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14657-1-AP

Print datasheet

Synonyms

ESX homeobox 1, ESX1, ESX1L, ESX1R, ESXR1, Homeobox protein ESX1

验证数据展示

  • WB analysis of mouse brain using 14657-1-AP

    WB analysis of mouse brain using 14657-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14657-1-AP (ESX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14657-1-AP

    IP experiment of mouse brain using 14657-1-AP

    IP result of anti-ESX1 (IP:14657-1-AP, 4ug; Detection:14657-1-AP 1:500) with mouse brain tissue lysate 3440ug.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14657-1-AP targets ESX1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ESX1 fusion protein Ag6269 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ESX homeobox 1
Synonyms ESX homeobox 1, ESX1, ESX1L, ESX1R, ESXR1, Homeobox protein ESX1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC053599
Gene Symbol ESX1
Gene ID (NCBI) 80712
RRIDAB_10694138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N693
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ESX1, also named as ESX1L and ESX1R , is an X-linked homeobox gene primarily expressed in the placenta and testis, with physiological functions in placenta/fetus development and spermatogenesis. It has a substantively different C-terminal region subject to strong purifying selection. ESX1 can be cleaved into 2 part: ESXR1-N(45kd) and ESXR1-C(20kd). ESXR1-N acts as a transcriptional repressor. ESXR1-C has the ability to inhibit cyclin turnover.

实验方案

Product Specific Protocols
WB protocol for ESX1 antibody 14657-1-APDownload protocol
IP protocol for ESX1 antibody 14657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols