验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IF | See 2 publications below |
产品信息
17925-1-AP targets ETFB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ETFB fusion protein Ag12344 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | electron-transfer-flavoprotein, beta polypeptide |
| 别名 | MADD, FP585, Beta-ETF |
| 计算分子量 | 255 aa, 28 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC093961 |
| 基因名称 | ETFB |
| Gene ID (NCBI) | 2109 |
| RRID | AB_2100560 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P38117 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kDa alpha subunits (ETFA)and 28 kDa beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer.It serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.ETFB has 2 isoforms produced by alternative splicing. Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETFB antibody 17925-1-AP | Download protocol |
| IHC protocol for ETFB antibody 17925-1-AP | Download protocol |
| IF protocol for ETFB antibody 17925-1-AP | Download protocol |
| IP protocol for ETFB antibody 17925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Mol Cell Biol A mutation in the FAD-dependent oxidoreductase FOXRED1 results in cell-type specific assembly defects in oxidative phosphorylation complexes I and II. | ||
J Biol Chem Human METTL20 Methylates Lysine Residues Adjacent to the Recognition Loop of the Electron Transfer Flavoprotein in Mitochondria. | ||
J Cell Sci Mitochondrial maintenance under oxidative stress depends on mitochondrial but not nuclear α isoform of OGG1. | ||
J Cell Mol Med New protein-protein interactions of mitochondrial connexin 43 in mouse heart. |
