验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10684-1-AP targets ETV4 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 | ETV4 fusion protein Ag0984 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ets variant 4 |
| 别名 | PEA3, ETS translocation variant 4, E1A-F, E1AF, Adenovirus E1A enhancer-binding protein |
| 计算分子量 | 54 kDa |
| 观测分子量 | 61-65 kDa |
| GenBank蛋白编号 | BC007242 |
| 基因名称 | ETV4 |
| Gene ID (NCBI) | 2118 |
| RRID | AB_2100984 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43268 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETV4 antibody 10684-1-AP | Download protocol |
| IF protocol for ETV4 antibody 10684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription. | ||
Dev Cell ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization.
| ||
Nat Commun Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5. | ||
Proc Natl Acad Sci U S A Erk1/2 inactivation promotes a rapid redistribution of COP1 and degradation of COP1 substrates. | ||
Oncogene HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer
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