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ETV5 Monoclonal antibody, PBS Only (Capture)

ETV5 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4A4

Cat No : 66657-1-PBS

Print datasheet

Synonyms

ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5

验证数据展示

  • WB analysis using 66657-1-Ig (same clone as 66657-1-PBS)

    WB analysis using 66657-1-Ig (same clone as 66657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66657-1-Ig (ETV5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66657-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50024-1

    Sandwich ELISA standard curve of MP50024-1

    Sandwich ELISA standard curve ofMP50024-1, ETV5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66657-1-PBS. Detection antibody: HRP-conjugated 66657-2-PBS. Standard: Ag3708. Range: 0.313-20 ng/m.

Planning an IHC experiment? We recommend our IHCeasy ETV5 Ready-To-Use IHC Kit. ETV5 primary antibody included.

产品信息

66657-1-PBS targets ETV5 as part of a matched antibody pair:

MP50024-1: 66657-1-PBS capture and 66657-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ETV5 fusion protein Ag3708 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ets variant 5
Synonyms ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5
Calculated Molecular Weight 510 aa, 58 kDa
Observed Molecular Weight 58-60 kDa
GenBank Accession NumberBC007333
Gene Symbol ETV5
Gene ID (NCBI) 2119
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41161
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

背景介绍

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney