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EVI1 Polyclonal antibody

EVI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 23201-1-AP

Print datasheet

Synonyms

MECOM, MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367

验证数据展示

  • WB analysis of SKOV-3 using 23201-1-AP

    WB analysis of SKOV-3 using 23201-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 23201-1-AP

    IHC staining of human thyroid cancer using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IHC staining of human thyroid cancer using 23201-1-AP

    IHC staining of human thyroid cancer using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IHC staining of human urothelial carcinoma using 23201-1-AP

    IHC staining of human urothelial carcinoma using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IHC staining of human urothelial carcinoma using 23201-1-AP

    IHC staining of human urothelial carcinoma using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IF Staining of SKOV-3 using 23201-1-AP

    IF Staining of SKOV-3 using 23201-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using EVI1 antibody (23201-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy EVI1 Ready-To-Use IHC Kit. EVI1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23201-1-AP targets EVI1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, CoIP, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen EVI1 fusion protein Ag19603 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ecotropic viral integration site 1
Synonyms MECOM, MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367
Calculated Molecular Weight 1239 aa, 139 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberBC130520
Gene Symbol EVI1
Gene ID (NCBI) 2122
RRIDAB_2879230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ03112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI1 antibody 23201-1-APDownload protocol
IHC protocol for EVI1 antibody 23201-1-APDownload protocol
IF protocol for EVI1 antibody 23201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Sci Adv

Predicting master transcription factors from pan-cancer expression data.

Authors - Jessica Reddy
View Article
humanCoIP

Oncol Rep

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis

Authors - Zhitao Chen
View Article