• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human brain using 17415-1-AP

    WB analysis of human brain using 17415-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17415-1-AP (EVI2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17415-1-AP

    IHC staining of human brain using 17415-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17415-1-AP (EVI2A antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17415-1-AP

    IHC staining of human brain using 17415-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17415-1-AP (EVI2A antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 17415-1-AP

    IF Staining of HepG2 using 17415-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EVI2A antibody (17415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EVI2A Polyclonal antibody

EVI2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17415-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

EVI-2A, EVI2, EVI 2A, EVDA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17415-1-AP targets EVI2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 EVI2A fusion protein Ag11075 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ecotropic viral integration site 2A
别名 EVI-2A, EVI2, EVI 2A, EVDA
计算分子量 236 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC035572
基因名称 EVI2A
Gene ID (NCBI) 2123
RRIDAB_2101143
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22794
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI2A antibody 17415-1-APDownload protocol
IHC protocol for EVI2A antibody 17415-1-APDownload protocol
IF protocol for EVI2A antibody 17415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

A combined omics approach to generate the surface atlas of human naive CD4+ T cells during early TCR activation.

Authors - Anke Graessel
View Article
humanWB

Oncol Res

Integrative bioinformatics and in vitro exploration of EVI2A expression: unraveling its immunological and prognostic implications in kidney renal clear cell carcinoma

Authors - Rong Liu
View Article