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验证数据展示

  • WB analysis of human brain using 17415-1-AP

    WB analysis of human brain using 17415-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17415-1-AP (EVI2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17415-1-AP

    IHC staining of human brain using 17415-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17415-1-AP (EVI2A antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17415-1-AP

    IHC staining of human brain using 17415-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17415-1-AP (EVI2A antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 17415-1-AP

    IF Staining of HepG2 using 17415-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EVI2A antibody (17415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EVI2A Polyclonal antibody

EVI2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17415-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EVI-2A, EVI2, EVI 2A, EVDA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17415-1-AP targets EVI2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen EVI2A fusion protein Ag11075 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ecotropic viral integration site 2A
Synonyms EVI-2A, EVI2, EVI 2A, EVDA
Calculated Molecular Weight 236 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC035572
Gene Symbol EVI2A
Gene ID (NCBI) 2123
RRIDAB_2101143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22794
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI2A antibody 17415-1-APDownload protocol
IHC protocol for EVI2A antibody 17415-1-APDownload protocol
IF protocol for EVI2A antibody 17415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

A combined omics approach to generate the surface atlas of human naive CD4+ T cells during early TCR activation.

Authors - Anke Graessel
View Article