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验证数据展示

  • WB analysis of HepG2 using 24891-1-AP

    WB analysis of HepG2 using 24891-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24891-1-AP (EVI2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 24891-1-AP

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI2B antibody included
  • IHC staining of human tonsillitis using 24891-1-AP

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI2B antibody included

EVI2B Polyclonal antibody

EVI2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24891-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Protein EVI2B, EVI-2B, EVI 2B, EVDB, D17S376

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EVI2B Ready-To-Use IHC Kit. EVI2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

24891-1-AP targets EVI2B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 EVI2B fusion protein Ag19511 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ecotropic viral integration site 2B
别名 Protein EVI2B, EVI-2B, EVI 2B, EVDB, D17S376
计算分子量 448 aa, 49 kDa
观测分子量 65 kDa
GenBank蛋白编号BC005926
基因名称 EVI2B
Gene ID (NCBI) 2124
RRIDAB_2879781
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP34910
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI2B antibody 24891-1-APDownload protocol
IHC protocol for EVI2B antibody 24891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Oncol Lett

Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma.

Authors - Bingsheng Yang
View Article
humanIHC

Exp Biol Med (Maywood)

Analysis of scRNA-seq and bulk RNA-seq demonstrates the effects of EVI2B or CD361 on CD8+ T cells in osteosarcoma

Authors - Tianyu Xie
View Article