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EVI2B Polyclonal antibody

EVI2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24891-1-AP

Print datasheet

Synonyms

Protein EVI2B, EVI-2B, EVI 2B, EVDB, D17S376

验证数据展示

  • WB analysis of HepG2 using 24891-1-AP

    WB analysis of HepG2 using 24891-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24891-1-AP (EVI2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 24891-1-AP

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI2B antibody included
  • IHC staining of human tonsillitis using 24891-1-AP

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI2B antibody included

经过测试的应用

Positive WB detected inHepG2 cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EVI2B Ready-To-Use IHC Kit. EVI2B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

24891-1-AP targets EVI2B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen EVI2B fusion protein Ag19511 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ecotropic viral integration site 2B
Synonyms Protein EVI2B, EVI-2B, EVI 2B, EVDB, D17S376
Calculated Molecular Weight 448 aa, 49 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC005926
Gene Symbol EVI2B
Gene ID (NCBI) 2124
RRIDAB_2879781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI2B antibody 24891-1-APDownload protocol
IHC protocol for EVI2B antibody 24891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Oncol Lett

Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma.

Authors - Bingsheng Yang
View Article
humanIHC

Exp Biol Med (Maywood)

Analysis of scRNA-seq and bulk RNA-seq demonstrates the effects of EVI2B or CD361 on CD8+ T cells in osteosarcoma

Authors - Tianyu Xie
View Article