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验证数据展示

  • IF Staining of U2OS using 84738-2-RR

    IF Staining of U2OS using 84738-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EVI5 antibody (84738-2-RR, Clone: 241918D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84738-2-RR

    Affinity and Kinetic Characterization of 84738-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84738-2-RR against Human EVI5 were performed. The affinity constant is 1.11 nM.

EVI5 Recombinant antibody

EVI5 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84738-2-RR

产品说明书

CloneNo. 241918D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

NB4S, 241918D11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84738-2-RR targets EVI5 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 EVI5 fusion protein Ag26739 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ecotropic viral integration site 5
别名 NB4S, 241918D11
计算分子量 810 aa, 93 kDa
GenBank蛋白编号BC156793
基因名称 EVI5
Gene ID (NCBI) 7813
RRIDAB_3672122
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60447
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EVI5 functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. It may play a role in cytokinesis (PMID: 16439210).

实验方案

Product Specific Protocols
IF protocol for EVI5 antibody 84738-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols