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EVL Polyclonal antibody

EVL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13484-1-AP

Print datasheet

Synonyms

Ena/VASP like protein, Enah/Vasp like, EVL, RNB6

验证数据展示

  • WB analysis of Jurkat using 13484-1-AP

    WB analysis of Jurkat using 13484-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13484-1-AP (EVL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 13484-1-AP

    IHC staining of human small intestine using 13484-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human small intestine using 13484-1-AP

    IHC staining of human small intestine using 13484-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman small intestine tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13484-1-AP targets EVL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen EVL fusion protein Ag4302 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Enah/Vasp-like
Synonyms Ena/VASP like protein, Enah/Vasp like, EVL, RNB6
Calculated Molecular Weight 418 aa, 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC032358
Gene Symbol EVL
Gene ID (NCBI) 51466
RRIDAB_10644121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI08
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EVL (Ena/VASP-like protein) is implicated in processes that requires dynamic actin remodeling such as axon guidance and platelet activation.It has two isoforms, EVL and EVL-I,that are highly expressed in hematopoietic cells of thymus and spleen,and both isoforms can be phosphorylated by PKA in vitro(PMID10945997).

实验方案

Product Specific Protocols
WB protocol for EVL antibody 13484-1-APDownload protocol
IHC protocol for EVL antibody 13484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Pers Med

Immunocyte Infiltration Analysis and Immunohistochemistry Identify EVL as a Potential Prognostic Biomarker for Pancreatic Cancer

Authors - Yan Du
View Article
mouseIHC

Clin Transl Med

Genetic and pharmacological inhibition of METTL3 alleviates renal fibrosis by reducing EVL m6A modification through an IGF2BP2-dependent mechanism

Authors - Wei-Jian Ni
View Article