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验证数据展示

  • IHC staining of human testis using 17313-1-AP

    IHC staining of human testis using 17313-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 17313-1-AP

    IHC staining of human testis using 17313-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of HeLa using 17313-1-AP

    WB analysis of HeLa using 17313-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 17313-1-AP

    WB analysis of SH-SY5Y using 17313-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17313-1-AP

    IF Staining of HeLa using 17313-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXD1 antibody (17313-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

EXD1 Polyclonal antibody

EXD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17313-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

piRNA biogenesis protein EXD1, Inactive exonuclease EXD1, Exonuclease 3'-5' domain-like-containing protein 1, Exonuclease 3'-5' domain-containing protein 1, EXDL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17313-1-AP targets EXD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性mouse
免疫原 EXD1 fusion protein Ag11282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exonuclease 3'-5' domain containing 1
别名 piRNA biogenesis protein EXD1, Inactive exonuclease EXD1, Exonuclease 3'-5' domain-like-containing protein 1, Exonuclease 3'-5' domain-containing protein 1, EXDL1
计算分子量 514 aa, 58 kDa
观测分子量 36 kDa, 58 kDa, 75 kDa
GenBank蛋白编号BC030628
基因名称 EXD1
Gene ID (NCBI) 161829
RRIDAB_2878379
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NHP7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXD1, also named as EXDL1, has three isoforms with MW 58 kDa, 36 kDa and 65 kDa. This antibody can recognize all these three isoforms.

实验方案

Product Specific Protocols
WB protocol for EXD1 antibody 17313-1-APDownload protocol
IHC protocol for EXD1 antibody 17313-1-APDownload protocol
IF protocol for EXD1 antibody 17313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Andrology

Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes

Authors - Opeyemi Olotu
View Article