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  • Featured Product
  • KD/KO Validated

EXD2 Polyclonal antibody

EXD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20138-1-AP

Print datasheet

Synonyms

C14orf114, EXD2, EXDL2

验证数据展示

  • WB analysis of mouse lung using 20138-1-AP

    WB analysis of mouse lung using 20138-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20138-1-AP

    IHC staining of human testis using 20138-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 20138-1-AP

    IF Staining of HepG2 using 20138-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EXD2 antibody (20138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse lung using 20138-1-AP

    IP experiment of mouse lung using 20138-1-AP

    IP result of anti-EXD2 (IP:20138-1-AP, 3ug; Detection:20138-1-AP 1:300) with mouse lung tissue lysate 5160ug.

经过测试的应用

Positive WB detected inmouse lung tissue, mouse brain tissue, mouse pancreas tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20138-1-AP targets EXD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen EXD2 fusion protein Ag14090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exonuclease 3'-5' domain containing 2
Synonyms C14orf114, EXD2, EXDL2
Calculated Molecular Weight 70 kD aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC001962
Gene Symbol EXD2
Gene ID (NCBI) 55218
RRIDAB_10665419
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EXD2 antibody 20138-1-APDownload protocol
IHC protocol for EXD2 antibody 20138-1-APDownload protocol
IF protocol for EXD2 antibody 20138-1-APDownload protocol
IP protocol for EXD2 antibody 20138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination.

Authors - Xikui Sun
  • KO Validated
View Article
mouseWB,IF

Andrology

Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes

Authors - Opeyemi Olotu
View Article