验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, Jurkat cells, HeLa cells, Raji cells |
| Positive IP detected in | mouse thymus tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16253-1-AP targets EXO1 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC, ChIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | EXO1 fusion protein Ag9253 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | exonuclease 1 |
| Synonyms | EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI |
| Calculated Molecular Weight | 94 kDa |
| Observed Molecular Weight | 115-120 kDa |
| GenBank Accession Number | BC007491 |
| Gene Symbol | EXO1 |
| Gene ID (NCBI) | 9156 |
| RRID | AB_2278140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQ84 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXO1 antibody 16253-1-AP | Download protocol |
| IP protocol for EXO1 antibody 16253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway. | ||
Elife Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations.
| ||
PLoS Genet EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair. | ||
J Cardiovasc Transl Res Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia. | ||
Front Genet Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.
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