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EXO1 Monoclonal antibody

EXO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11D1

Cat no : 68536-1-Ig

Print datasheet

Synonyms

EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI

验证数据展示

  • WB analysis using 68536-1-Ig

    WB analysis using 68536-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68536-1-Ig (EXO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, HEK-293T cells, MOLT-4 cells, TF-1 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68536-1-Ig targets EXO1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen EXO1 fusion protein Ag9253 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name exonuclease 1
Synonyms EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC007491
Gene Symbol EXO1
Gene ID (NCBI) 9156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UQ84
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.

实验方案

Product Specific Protocols
WB protocol for EXO1 antibody 68536-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols