验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, COLO 320 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14703-1-AP targets SEC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | SEC6 fusion protein Ag6383 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exocyst complex component 3 |
| 别名 | EXOC3, SEC6L1, Exocyst complex component Sec6, Exocyst complex component 3 |
| 计算分子量 | 87 kDa |
| 观测分子量 | 86 kDa |
| GenBank蛋白编号 | BC064569 |
| 基因名称 | SEC6 |
| Gene ID (NCBI) | 11336 |
| RRID | AB_2101542 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60645 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC6 antibody 14703-1-AP | Download protocol |
| IHC protocol for SEC6 antibody 14703-1-AP | Download protocol |
| IF protocol for SEC6 antibody 14703-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Microbiol Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells.
| ||
Cell Microbiol Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway.
| ||
Dev Biol Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition. | ||
Pathol Res Pract Exosome secretion related gene signature predicts chemoresistance in patients with colorectal cancer | ||
