验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse testis tissue, U-251 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
11913-1-AP targets Sec8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Sec8 fusion protein Ag2514 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exocyst complex component 4 |
| 别名 | SEC8L1, REC8, KIAA1699, Exocyst complex component Sec8, Exocyst complex component 4 |
| 计算分子量 | 110 kDa |
| 观测分子量 | 110-120 kDa |
| GenBank蛋白编号 | BC026174 |
| 基因名称 | Sec8 |
| Gene ID (NCBI) | 60412 |
| RRID | AB_2101565 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96A65 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Sec8 antibody 11913-1-AP | Download protocol |
| IHC protocol for Sec8 antibody 11913-1-AP | Download protocol |
| IP protocol for Sec8 antibody 11913-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. | ||
Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis | ||
Biol Res REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells. | ||
Commun Biol The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium |
