验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human adrenal gland tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy EXOC5 Ready-To-Use IHC Kit. EXOC5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17593-1-AP targets EXOC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | EXOC5 fusion protein Ag11524 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exocyst complex component 5 |
| 别名 | Exocyst complex component 5, Exocyst complex component Sec10, hSec10, SEC10, SEC10L1 |
| 计算分子量 | 708 aa, 82 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC041126 |
| 基因名称 | EXOC5 |
| Gene ID (NCBI) | 10640 |
| RRID | AB_2101582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00471 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXOC5 (SEC10), a component of the exocyst complex, is a 77-kDa protein with a broad tissue distribution (PubMed: 9119050). The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| IHC protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| IF protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| IP protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly.
| ||
Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis | ||
Mol Cancer Res EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.
| ||
Cells The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells | ||
J Biol Chem Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model.
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