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验证数据展示

  • IP experiment of HEK-293 using 55440-1-AP

    IP experiment of HEK-293 using 55440-1-AP

    IP result of anti-EXOC6B (IP:55440-1-AP, 4ug; Detection:55440-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • IF Staining of HEK-293 using 55440-1-AP

    IF Staining of HEK-293 using 55440-1-AP

    Immunofluorescent analysis of HEK-293 cells using 55440-1-AP (EXOC6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 55440-1-AP

    IHC staining of human liver using 55440-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 55440-1-AP

    IHC staining of human liver using 55440-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 40x lens).

EXOC6B Polyclonal antibody

EXOC6B Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 55440-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IP, ELISA

别名

EXOC6B, exocyst complex component 6B, KIAA0919, SEC15 like protein 2, SEC15B, SEC15L2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55440-1-AP targets EXOC6B in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exocyst complex component 6B
别名 EXOC6B, exocyst complex component 6B, KIAA0919, SEC15 like protein 2, SEC15B, SEC15L2
计算分子量 94 kDa
观测分子量 94 kDa
GenBank蛋白编号NM_015189
基因名称 EXOC6B
Gene ID (NCBI) 23233
RRIDAB_2881333
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2D4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for EXOC6B antibody 55440-1-APDownload protocol
IF protocol for EXOC6B antibody 55440-1-APDownload protocol
IP protocol for EXOC6B antibody 55440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols