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EXOC6B Polyclonal antibody

EXOC6B Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 55440-1-AP

Print datasheet

Synonyms

EXOC6B, exocyst complex component 6B, KIAA0919, SEC15 like protein 2, SEC15B, SEC15L2

验证数据展示

  • IP experiment of HEK-293 using 55440-1-AP

    IP experiment of HEK-293 using 55440-1-AP

    IP result of anti-EXOC6B (IP:55440-1-AP, 4ug; Detection:55440-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • IF Staining of HEK-293 using 55440-1-AP

    IF Staining of HEK-293 using 55440-1-AP

    Immunofluorescent analysis of HEK-293 cells using 55440-1-AP (EXOC6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 55440-1-AP

    IHC staining of human liver using 55440-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 55440-1-AP

    IHC staining of human liver using 55440-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55440-1-AP (EXOC6B antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55440-1-AP targets EXOC6B in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exocyst complex component 6B
Synonyms EXOC6B, exocyst complex component 6B, KIAA0919, SEC15 like protein 2, SEC15B, SEC15L2
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberNM_015189
Gene Symbol EXOC6B
Gene ID (NCBI) 23233
RRIDAB_2881333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2D4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for EXOC6B antibody 55440-1-APDownload protocol
IF protocol for EXOC6B antibody 55440-1-APDownload protocol
IP protocol for EXOC6B antibody 55440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols