验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14805-1-AP targets EXOSC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | EXOSC2 fusion protein Ag6525 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exosome component 2 |
| 别名 | Rrp4p, RRP4, Ribosomal RNA-processing protein 4, hRrp4p, Exosome component 2 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 30-33 kDa |
| GenBank蛋白编号 | BC000747 |
| 基因名称 | EXOSC2 |
| Gene ID (NCBI) | 23404 |
| RRID | AB_2101837 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13868 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC2 antibody 14805-1-AP | Download protocol |
| IHC protocol for EXOSC2 antibody 14805-1-AP | Download protocol |
| IF protocol for EXOSC2 antibody 14805-1-AP | Download protocol |
| IP protocol for EXOSC2 antibody 14805-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression. | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
Mol Biotechnol EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal
| ||
Mol Cell The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex | ||
