验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | 4T1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11979-1-AP targets EXOSC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | EXOSC8 fusion protein Ag2605 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exosome component 8 |
| 别名 | RRP43, Ribosomal RNA-processing protein 43, p9, Opa-interacting protein 2, OIP2 |
| 计算分子量 | 276 aa, 30 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC020773 |
| 基因名称 | EXOSC8 |
| Gene ID (NCBI) | 11340 |
| RRID | AB_2101981 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96B26 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IHC protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IF protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IP protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.
| ||
Nat Commun EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.
| ||
PLoS One Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA. | ||
Neuromuscul Disord New subtype of PCH1C caused by novel EXOSC8 variants in a 16-year-old Spanish patient. | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
