验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | 4T1 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11979-1-AP targets EXOSC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | EXOSC8 fusion protein Ag2605 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | exosome component 8 |
| Synonyms | RRP43, Ribosomal RNA-processing protein 43, p9, Opa-interacting protein 2, OIP2 |
| Calculated Molecular Weight | 276 aa, 30 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC020773 |
| Gene Symbol | EXOSC8 |
| Gene ID (NCBI) | 11340 |
| RRID | AB_2101981 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96B26 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IHC protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IF protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| IP protocol for EXOSC8 antibody 11979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.
| ||
Nat Commun EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.
| ||
PLoS One Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA. | ||
Neuromuscul Disord New subtype of PCH1C caused by novel EXOSC8 variants in a 16-year-old Spanish patient. | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
