验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy EXOSC9 Ready-To-Use IHC Kit. EXOSC9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
24470-1-AP targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | EXOSC9 fusion protein Ag19768 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exosome component 9 |
| 别名 | PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45 |
| 计算分子量 | 456 aa, 51 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC142978 |
| 基因名称 | EXOSC9 |
| Gene ID (NCBI) | 5393 |
| RRID | AB_2879559 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q06265 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| IHC protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| IF protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
