验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22199-1-AP targets EXPH5 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | EXPH5 fusion protein Ag17449 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exophilin 5 |
| 别名 | exophilin 5, EXPH5, KIAA0624, SLAC2 B |
| 计算分子量 | 1989 aa, 223 kDa |
| 观测分子量 | 222-240 kDa |
| GenBank蛋白编号 | BC113119 |
| 基因名称 | EXPH5 |
| Gene ID (NCBI) | 23086 |
| RRID | AB_2879026 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q8NEV8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXPH5, also named as KIAA0624 or SLAC2B, is a 1989 amino acid protein, which contains one RabBD (Rab-binding) domain. EXPH5 is expressed in keratinocytes. EXPH5 may act as Rab effector protein and play a role in vesicle trafficking. EXPH5 plays a role in maintaining keratinocyte integrity, and in inherited skin fragility.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXPH5 antibody 22199-1-AP | Download protocol |
| IHC protocol for EXPH5 antibody 22199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
