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验证数据展示

  • WB analysis using 11348-1-AP

    WB analysis using 11348-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11348-1-AP

    IHC staining of human colon cancer using 11348-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11348-1-AP (EXT2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of Jurkat using 11348-1-AP

    IF Staining of Jurkat using 11348-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EXT2 Polyclonal antibody

EXT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11348-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Multiple exostoses protein 2, Heparan sulfate co-polymerase subunit EXT1, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, EXT-2, Exostosin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells, U-937 cells, C6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11348-1-AP targets EXT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 EXT2 fusion protein Ag1748 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exostoses (multiple) 2
别名 Multiple exostoses protein 2, Heparan sulfate co-polymerase subunit EXT1, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, EXT-2, Exostosin 2
计算分子量 718 aa, 82 kDa
观测分子量 70-82 kDa
GenBank蛋白编号BC010058
基因名称 EXT2
Gene ID (NCBI) 2132
RRIDAB_2293839
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93063
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Exostosin-2 (EXT2) is type II transmembrane glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone(PMID: 22660413, 36402845, 36593275).

实验方案

Product Specific Protocols
WB protocol for EXT2 antibody 11348-1-APDownload protocol
IHC protocol for EXT2 antibody 11348-1-APDownload protocol
IF protocol for EXT2 antibody 11348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome

Authors - Valeria De Pasquale
View Article