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EXTL2 Polyclonal antibody

EXTL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12828-1-AP

Print datasheet

Synonyms

Exostosin like 2, exostoses (multiple) like 2, EC:2.4.1.223, Alpha-GalNAcT EXTL2, Alpha-1,4-N-acetylhexosaminyltransferase EXTL2

验证数据展示

  • WB analysis using 12828-1-AP

    WB analysis using 12828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12828-1-AP (EXTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 12828-1-AP

    IF Staining of SH-SY5Y using 12828-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EXTL2 antibody (12828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inSH-SY5Y cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12828-1-AP targets EXTL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EXTL2 fusion protein Ag3548 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exostoses (multiple)-like 2
Synonyms Exostosin like 2, exostoses (multiple) like 2, EC:2.4.1.223, Alpha-GalNAcT EXTL2, Alpha-1,4-N-acetylhexosaminyltransferase EXTL2
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight 37-43 kDa
GenBank Accession NumberBC036015
Gene Symbol EXTL2
Gene ID (NCBI) 2135
RRIDAB_2102130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBQ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Exostosin-like 2 (EXTL2) is one of three EXT-like genes in the human genome that are homologous to EXT1 and EXT2, encodes a transferase that adds not only GlcNAc but also N-acetylgalactosamine to the glycosaminoglycan (GAG)-protein linkage region via an α1,4-linkage. Loss of the enzyme EXTL2, a regulatory mechanism serving to limit CSPG synthesis, resulted in more extensive versican deposition into a lysolecithin-induced demyelinated injury, a larger area of myelin disruption with an associated decrease in axonal density within the lesion, and greater accumulation of macrophages and microglia (PMID: 32703234, 23395820)

实验方案

Product Specific Protocols
WB protocol for EXTL2 antibody 12828-1-APDownload protocol
IF protocol for EXTL2 antibody 12828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols