EXTL3 Polyclonal antibody

EXTL3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 10588-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

IHC, IF/ICC, ELISA and More (2)

Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, EXT-related protein 1, EXTR1, EXTL1L, Exostosin-like 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10588-1-AP targets EXTL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 EXTL3 fusion protein Ag0936 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exostoses (multiple)-like 3
别名 Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, EXT-related protein 1, EXTR1, EXTL1L, Exostosin-like 3
计算分子量 105 kDa
观测分子量 65 kDa
GenBank蛋白编号BC006363
基因名称 EXTL3
Gene ID (NCBI) 2137
RRIDAB_2262577
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43909
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Exostosin like glycosyltransferase 3 (EXTL3), also known as RPR, is located at chromosome 8p21.1 and composed of 919 amino acids, making it the longest member of the EXT family (PMID: 35676258). EXTL3 encodes the glycosyltransferases responsible for the biosynthesis of the backbone structure of heparan sulfate (HS) (PMID: 24128412). EXTL3 has been identified as a receptor molecule for regenerating islet-derived (REG) protein ligands, which have been shown to stimulate islet β-cell growth. Mutations in EXTL3 could lead to skeletal dysplasia, developmental delay, and severe immune deficiency (PMID: 28148688). The predicted molecular weight is 105 kDa, but we detected the band at 65 kDa perhaps due to alternative splicing (PMID: 14618397).

实验方案

Product Specific Protocols
IHC protocol for EXTL3 antibody 10588-1-APDownload protocol
IF protocol for EXTL3 antibody 10588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Endocr Pract

Discovery of a human peptide sequence signaling islet neogenesis.

Authors - Levetan Claresa S CS
human,mouseWB,IF

Ann N Y Acad Sci

REG1A protects retinal photoreceptors from blue light damage

Authors - Ze-Hua Xu
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