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EYA1 Polyclonal antibody

EYA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31435-1-AP

Print datasheet

Synonyms

BOP, BOR, EYA1, Eyes absent homolog 1

验证数据展示

  • WB analysis using 31435-1-AP

    WB analysis using 31435-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31435-1-AP (EYA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNCCIT cells, mouse kidney tissue, rat kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31435-1-AP targets EYA1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen EYA1 fusion protein Ag33438 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eyes absent homolog 1 (Drosophila)
Synonyms BOP, BOR, EYA1, Eyes absent homolog 1
Calculated Molecular Weight 592 aa, 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC121799
Gene Symbol EYA1
Gene ID (NCBI) 2138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ99502
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).

实验方案

Product Specific Protocols
WB protocol for EYA1 antibody 31435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols