验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Y79 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy EYA2 Ready-To-Use IHC Kit. EYA2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11314-1-AP targets EYA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | EYA2 fusion protein Ag1860 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eyes absent homolog 2 (Drosophila) |
| 别名 | EC:3.1.3.48, EAB1 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 56 kDa and 70 kDa |
| GenBank蛋白编号 | BC013882 |
| 基因名称 | EYA2 |
| Gene ID (NCBI) | 2139 |
| RRID | AB_10733651 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00167 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. It can be detected two bands of 70 kDa and 56 kDa in the neuroblastoma cells by western blot(PMID:12039049).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EYA2 antibody 11314-1-AP | Download protocol |
| IHC protocol for EYA2 antibody 11314-1-AP | Download protocol |
| IF protocol for EYA2 antibody 11314-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-β signaling, epithelial-mesenchymal transition, and cancer stem cell properties. | ||
Front Cell Dev Biol A Phosphotyrosine Switch in Estrogen Receptor β Is Required for Mouse Ovarian Function. | ||
Int J Mol Med Eya2 overexpression promotes the invasion of human astrocytoma through the regulation of ERK/MMP9 signaling. | ||
Stem Cells Int Noggin Combined With Human Dental Pulp Stem Cells to Promote Skeletal Muscle Regeneration |