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验证数据展示

  • IHC staining of mouse eye using 30607-1-AP

    IHC staining of mouse eye using 30607-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 30607-1-AP (EYA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EYA2 antibody included
  • IHC staining of mouse eye using 30607-1-AP

    IHC staining of mouse eye using 30607-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 30607-1-AP (EYA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EYA2 antibody included
  • IF Staining of HeLa using 30607-1-AP

    IF Staining of HeLa using 30607-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA2 antibody (30607-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

EYA2 Polyclonal antibody

EYA2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30607-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

EC:3.1.3.48, EAB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EYA2 Ready-To-Use IHC Kit. EYA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30607-1-AP targets EYA2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 EYA2 fusion protein Ag31827 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eyes absent homolog 2 (Drosophila)
别名 EC:3.1.3.48, EAB1
计算分子量 64 kDa
GenBank蛋白编号BC013882
基因名称 EYA2
Gene ID (NCBI) 2139
RRIDAB_3669738
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00167
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. It can be detected two bands of 70 kDa and 56 kDa in the neuroblastoma cells by western blot(PMID:12039049).

实验方案

Product Specific Protocols
IHC protocol for EYA2 antibody 30607-1-APDownload protocol
IF protocol for EYA2 antibody 30607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols