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验证数据展示

  • WB analysis using 84824-2-RR (same clone as 84824-2-PBS)

    WB analysis using 84824-2-RR (same clone as 84824-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84824-2-RR (EZH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84824-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84824-2-RR (same clone as 84824-2-PBS)

    IF Staining of HepG2 using 84824-2-RR (same clone as 84824-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-2-RR, Clone: 242309F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84824-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01605-1

    Cytometric bead array standard curve of MP01605-1

    Cytometric bead array standard curve of MP01605-1, EZH2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84824-2-PBS. Detection antibody: 84824-3-PBS. Standard: Ag16789. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01605-2

    Cytometric bead array standard curve of MP01605-2

    Cytometric bead array standard curve of MP01605-2, EZH2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84824-2-PBS. Detection antibody: 84824-1-PBS. Standard: Ag16789. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84824-2-RR

    Affinity and Kinetic Characterization of 84824-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84824-2-RR against Human EZH2 were performed. The affinity constant is 2.73 nM.

EZH2 Recombinant antibody, PBS Only (Capture)

EZH2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84824-2-PBS

产品说明书

CloneNo. 242309F11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84824-2-PBS targets EZH2 as part of a matched antibody pair:

MP01605-1: 84824-2-PBS capture and 84824-3-PBS detection (validated in Cytometric bead array)

MP01605-2: 84824-2-PBS capture and 84824-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EZH2 fusion protein Ag16789 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 enhancer of zeste homolog 2 (Drosophila)
别名 EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356
计算分子量 751 aa, 86 kDa
观测分子量 90-102 kDa
GenBank蛋白编号BC010858
基因名称 EZH2
Gene ID (NCBI) 2146
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15910
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)