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验证数据展示

  • WB analysis using 30847-1-AP

    WB analysis using 30847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30847-1-AP (Egfr antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30847-1-AP

    IF Staining of A431 using 30847-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Egfr antibody (30847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EGFR Polyclonal antibody

EGFR Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30847-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

HER1, Errp, Erbb1, erbb 1, Erbb

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, mouse liver tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30847-1-AP targets EGFR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EGFR fusion protein Ag33621 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor
别名 HER1, Errp, Erbb1, erbb 1, Erbb
计算分子量135 kDa
观测分子量140-180 kDa
GenBank蛋白编号NM_207655
基因名称 Egfr
Gene ID (NCBI) 13649
RRIDAB_3086421
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ01279
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The epidermal growth factor receptor (EGFR) is a member of the ErbB family of receptor tyrosine kinases (RTKs). Binding of EGFR to epidermal growth factor family ligands triggers a signaling cascade through allosteric activation of epidermal growth factor receptor kinase and trans-autophosphorylation of key tyrosine residues in the tail of the cytoplasmic receptor (PMID: 24691965). Due to phosphorylation, the apparent molecular weight is higher than the calculated molecular weight of 140 kDa. EFGR has been implicated in learning and memory enhancement and pain signaling in mammals (PMID: 20639532 and 35131940).

实验方案

Product Specific Protocols
WB protocol for EGFR antibody 30847-1-APDownload protocol
IF protocol for EGFR antibody 30847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols