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Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230047E8

Cat no : 82993-5-RR

Print datasheet

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 230047E8

验证数据展示

  • IF Staining of HUVEC using 82993-5-RR

    IF Staining of HUVEC using 82993-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ENG antibody (82993-5-RR, Clone: 230047E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 82993-5-RR

    Affinity and Kinetic Characterization of 82993-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82993-5-RR against Human Endoglin/CD105 were performed. The affinity constant is 0.296 nM.

经过测试的应用

Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD105 Ready-To-Use IHC Kit. CD105 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82993-5-RR targets Endoglin/CD105 in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name endoglin
Synonyms CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 230047E8
Calculated Molecular Weight 71 kDa
GenBank Accession NumberBC014271
Gene Symbol CD105
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
IF protocol for Endoglin/CD105 antibody 82993-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols