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Endothelin 3 Polyclonal antibody

Endothelin 3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27871-1-AP

Print datasheet

Synonyms

EDN3, PPET3, ET-3, ET3, ET 3

验证数据展示

  • WB analysis using 27871-1-AP

    WB analysis using 27871-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27871-1-AP (Endothelin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 27871-1-AP

    IHC staining of human thyroid cancer using 27871-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 27871-1-AP

    IHC staining of human thyroid cancer using 27871-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 27871-1-AP

    IHC staining of human oesophagus cancer using 27871-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 27871-1-AP

    IHC staining of human oesophagus cancer using 27871-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 27871-1-AP

    IF Staining of NIH/3T3 using 27871-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman thyroid cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27871-1-AP targets Endothelin 3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Endothelin 3 fusion protein Ag27393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endothelin 3
Synonyms EDN3, PPET3, ET-3, ET3, ET 3
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC053866
Gene Symbol Endothelin 3
Gene ID (NCBI) 1908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14138
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endothelin 3 (EDN3) is also named as ET-3 and Preproendothelin-3 (PPET3). Endothelin 3 belongs to the endothelin/sarafotoxin family. It reported downregulation of EDN3 as a result of methylation of its promoter in breast cancer, and EDN3 level was also decreased in cervical cancer (PMID: 36542159). ET-3 is the most abundant ET peptide in the rat brain, mainly localized to neurons and glia of the neostriatum, hypothalamic nuclei, hippocampus, and Purkinje cells of the cerebellum and medulla oblongata (PMID: 32589590).

实验方案

Product Specific Protocols
WB protocol for Endothelin 3 antibody 27871-1-APDownload protocol
IHC protocol for Endothelin 3 antibody 27871-1-APDownload protocol
IF protocol for Endothelin 3 antibody 27871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols