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Entactin Monoclonal antibody

Entactin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11G1

Cat no : 67310-1-Ig

Print datasheet

Synonyms

Entactin, NID, NID 1, NID1, nidogen 1

验证数据展示

  • WB analysis of mouse kidney using 67310-1-Ig

    WB analysis of mouse kidney using 67310-1-Ig

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 67310-1-Ig

    IHC staining of human ovary tumor using 67310-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 67310-1-Ig

    IHC staining of human ovary tumor using 67310-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67310-1-Ig targets Entactin in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Pig, Mouse, Rat
Immunogen Entactin fusion protein Ag4739 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nidogen 1
Synonyms Entactin, NID, NID 1, NID1, nidogen 1
Calculated Molecular Weight 1247 aa, 136 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC045606
Gene Symbol Entactin/NID
Gene ID (NCBI) 4811
RRIDAB_2882570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Entactin antibody 67310-1-IgDownload protocol
IHC protocol for Entactin antibody 67310-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols