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EPCAM/CD326 Recombinant antibody, PBS Only (Detector)

EPCAM/CD326 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241243E4

Cat no : 84073-4-PBS

Print datasheet

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

验证数据展示

  • Sandwich ELISA standard curve of MP01009-4

    Sandwich ELISA standard curve of MP01009-4

    Sandwich ELISA standard curve of MP01009-4, Human EPCAM/CD326 Recombinant Matched Antibody Pair - PBS only. 84073-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1370. 84073-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • Sample test of MP01009-4

    Sample test of MP01009-4

    The mean EPCAM/CD326 concentration was determined to be 492922.2 pg/mL in HT-116 cell extract based on a 2.0 mg/mL extract load.

  • Sample test of MP01009-4

    Sample test of MP01009-4

    HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human EPCAM/CD326, and measured 6718.5 pg/mL.

  • Sample test of MP01009-4

    Sample test of MP01009-4

    Serum of six individual healthy human donors was measured. The EPCAM/CD326 concentration of detected samples was determined to be 484.9 pg/mL with a range of 81.5-2002.2 pg/mL.

  • Cytometric bead array standard curve of MP01009-2

    Cytometric bead array standard curve of MP01009-2

    Cytometric bead array standard curve of MP01009-2, EPCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84073-5-PBS. Detection antibody: 84073-4-PBS. Standard: Eg1370. Range: 0.156-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.

产品信息

84073-4-PBS targets EPCAM/CD326 as part of a matched antibody pair:

MP01009-2: 84073-5-PBS capture and 84073-4-PBS detection (validated in Cytometric bead array)

MP01009-4: 84073-7-PBS capture and 84073-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_002354.3
Gene Symbol EPCAM
Gene ID (NCBI) 4072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16422
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C