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Host
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Species Reactivity
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Species Reactivity
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Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 21875-1-AP (same clone as 21875-1-PBS)

    WB analysis using 21875-1-AP (same clone as 21875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21875-1-AP (EphA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuron cells using 21875-1-AP (same clone as 21875-1-PBS)

    IF Staining of Neuron cells using 21875-1-AP (same clone as 21875-1-PBS)

    IF result of EphA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    IP experiment of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    IP result of anti-EphA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EphA4 Polyclonal antibody, PBS Only

EphA4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21875-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

21875-1-PBS targets EphA4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EphA4 fusion protein Ag16233 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EPH receptor A4
别名
计算分子量 986 aa, 110 kDa
观测分子量 120 kDa
GenBank蛋白编号BC026327
基因名称 EPHA4
Gene ID (NCBI) 2043
RRIDAB_2878936
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54764
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EphA4 is a member of the Eph receptor tyrosine kinase family and has important functions in the developing and adult nervous system (PMID: 14697664). The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). EphA4 is involved in commissure formation within the forebrain, axonal guidance in the corticospinal tract, regulation of the central pattern generator that provides normal locomotor function and axonal regeneration following spinal cord injury (PMID: 30061574). EphA4 has been implicated as a disease modifier of amyotrophic lateral sclerosis (ALS) (PMID: 22922411).