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Ephrin B2 Polyclonal antibody

Ephrin B2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26533-1-AP

Print datasheet

Synonyms

EFNB2, EPLG5, HTK ligand, Htk L, Ephrin-B2

验证数据展示

  • WB analysis using 26533-1-AP

    WB analysis using 26533-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26533-1-AP (Ephrin B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 26533-1-AP

    IF Staining of SH-SY5Y using 26533-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Ephrin B2 antibody (26533-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse lung tissue, mouse placenta tissue, mouse spleen tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26533-1-AP targets Ephrin B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Ephrin B2 fusion protein Ag24865 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ephrin-B2
Synonyms EFNB2, EPLG5, HTK ligand, Htk L, Ephrin-B2
Calculated Molecular Weight 333 aa, 37 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC074856
Gene Symbol EFNB2
Gene ID (NCBI) 1948
RRIDAB_3085878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52799
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730).

实验方案

Product Specific Protocols
WB protocol for Ephrin B2 antibody 26533-1-APDownload protocol
IF protocol for Ephrin B2 antibody 26533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols