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Ephrin B2 Polyclonal antibody

Ephrin B2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26533-1-AP

Print datasheet

Synonyms

EFNB2, ephrin B2, EPLG5, Htk L, HTK ligand, HTKL, LERK 5, LERK5

验证数据展示

  • WB analysis using 26533-1-AP

    WB analysis using 26533-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26533-1-AP (Ephrin B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse lung tissue, mouse placenta tissue, mouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26533-1-AP targets Ephrin B2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Ephrin B2 fusion protein Ag24865 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ephrin-B2
Synonyms EFNB2, ephrin B2, EPLG5, Htk L, HTK ligand, HTKL, LERK 5, LERK5
Calculated Molecular Weight 333 aa, 37 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC074856
Gene Symbol EFNB2
Gene ID (NCBI) 1948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52799
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730).

实验方案

Product Specific Protocols
WB protocol for Ephrin B2 antibody 26533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols