Various lysates were subjected to SDS PAGE followed by western blot with 84449-1-RR (XPO4 antibody) at dilution of 1:8500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

WB result of Exportin 4 antibody (84449-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Exportin 4 transfected HEK-293 cells. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Exportin 4 antibody (84449-1-RR, Clone: 241443A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug Exportin 4 Recombinant antibody (84449-1-RR, Clone:241443A5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 84449-1-RR against Human Exportin 4 were performed. The affinity constant is 0.512 nM.