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验证数据展示

  • IHC staining of human breast cancer using 26941-1-AP

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26941-1-AP

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 26941-1-AP

    WB analysis using 26941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

JAM-A/CD321 Polyclonal antibody

JAM-A/CD321 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26941-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

F11R, JAM-A/Junctional Adhesion Molecule A, JAM A, JAM 1, JAM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, human peripheral blood platelets, U-87 MG cells
Positive IHC detected inhuman breast cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26941-1-AP targets JAM-A/CD321 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 JAM-A/CD321 fusion protein Ag25641 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F11 receptor
别名 F11R, JAM-A/Junctional Adhesion Molecule A, JAM A, JAM 1, JAM
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001533
基因名称 F11R
Gene ID (NCBI) 50848
RRIDAB_2880692
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y624
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and ligand for the integrin LFA1.

实验方案

Product Specific Protocols
WB protocol for JAM-A/CD321 antibody 26941-1-APDownload protocol
IHC protocol for JAM-A/CD321 antibody 26941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols