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验证数据展示

  • WB analysis of human plasma using 27154-1-AP

    WB analysis of human plasma using 27154-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27154-1-AP

    IHC staining of human liver using 27154-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27154-1-AP

    IHC staining of human liver using 27154-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 40x lens).

Factor XII Polyclonal antibody

Factor XII Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27154-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

F12, HAE3, Factor 12, EC:3.4.21.38, Coagulation factor XII

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, K-562 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27154-1-AP targets Factor XII in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Factor XII fusion protein Ag25912 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coagulation factor XII (Hageman factor)
别名 F12, HAE3, Factor 12, EC:3.4.21.38, Coagulation factor XII
计算分子量 615 aa, 68 kDa
观测分子量 80 kDa and 52 kDa
GenBank蛋白编号BC012390
基因名称 Factor XII
Gene ID (NCBI) 2161
RRIDAB_2880778
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

实验方案

Product Specific Protocols
WB protocol for Factor XII antibody 27154-1-APDownload protocol
IHC protocol for Factor XII antibody 27154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols