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Factor XII Polyclonal antibody

Factor XII Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27154-1-AP

Print datasheet

Synonyms

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

验证数据展示

  • WB analysis of human plasma using 27154-1-AP

    WB analysis of human plasma using 27154-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27154-1-AP

    IHC staining of human liver using 27154-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27154-1-AP

    IHC staining of human liver using 27154-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inhuman plasma tissue, K-562 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27154-1-AP targets Factor XII in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Factor XII fusion protein Ag25912 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor XII (Hageman factor)
Synonyms Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight 80 kDa and 52 kDa
GenBank Accession NumberBC012390
Gene Symbol Factor XII
Gene ID (NCBI) 2161
RRIDAB_2880778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

实验方案

Product Specific Protocols
WB protocol for Factor XII antibody 27154-1-APDownload protocol
IHC protocol for Factor XII antibody 27154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols