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验证数据展示

  • WB analysis of mouse lung using 17223-1-AP

    WB analysis of mouse lung using 17223-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17223-1-AP (Factor XIIIa antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human normal colon using 17223-1-AP

    IHC staining of human normal colon using 17223-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17223-1-AP (Factor XIIIa antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with F13A1 antibody included

Factor XIIIa Polyclonal antibody

Factor XIIIa Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17223-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

F13A1, Transglutaminase A chain, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy F13A1 Ready-To-Use IHC Kit. F13A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17223-1-AP targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Factor XIIIa fusion protein Ag11059 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coagulation factor XIII, A1 polypeptide
别名 F13A1, Transglutaminase A chain, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13
计算分子量 732 aa, 83 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC027963
基因名称 Factor XIIIa
Gene ID (NCBI) 2162
RRIDAB_2878363
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00488
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.

实验方案

Product Specific Protocols
WB protocol for Factor XIIIa antibody 17223-1-APDownload protocol
IHC protocol for Factor XIIIa antibody 17223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols