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  • Featured Product
  • KD/KO Validated

PAR1/Thrombin Receptor Polyclonal antibody

PAR1/Thrombin Receptor Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26366-1-AP

Print datasheet

Synonyms

F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1

验证数据展示

  • WB analysis using 26366-1-AP

    WB analysis using 26366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26366-1-AP (PAR1/Thrombin Receptor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 26366-1-AP

    WB analysis of K-562 using 26366-1-AP

    WB result of F2R antibody (26366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAR1/Thrombin Receptor transfected K-562 cells.

  • IHC staining of human liver using 26366-1-AP

    IHC staining of human liver using 26366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26366-1-AP

    IHC staining of human liver using 26366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inY79 cells, K-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26366-1-AP targets PAR1/Thrombin Receptor in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human
Cited Reactivityhuman, mouse, hamster
Immunogen PAR1/Thrombin Receptor fusion protein Ag24378 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor II (thrombin) receptor
Synonyms F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC051909
Gene Symbol F2R
Gene ID (NCBI) 2149
RRIDAB_2880492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)

实验方案

Product Specific Protocols
WB protocol for PAR1/Thrombin Receptor antibody 26366-1-APDownload protocol
IHC protocol for PAR1/Thrombin Receptor antibody 26366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Breast Cancer Res Treat

Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.

Authors - Yihui Zhu
View Article
hamsterWB

Leukemia

Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15

Authors - Cong Liu
View Article