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F2RL3 Polyclonal antibody

F2RL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25306-1-AP

Print datasheet

Synonyms

Proteinase-activated receptor 4, Proteinase activated receptor 4, PAR-4, PAR4, PAR 4

验证数据展示

  • IHC staining of mouse lung using 25306-1-AP

    IHC staining of mouse lung using 25306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 25306-1-AP

    IHC staining of mouse lung using 25306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 25306-1-AP

    WB analysis using 25306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 25306-1-AP

    WB analysis of mouse lung using 25306-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse lung tissue, mouse pancreas tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

25306-1-AP targets F2RL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen F2RL3 fusion protein Ag20801 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor II (thrombin) receptor-like 3
Synonyms Proteinase-activated receptor 4, Proteinase activated receptor 4, PAR-4, PAR4, PAR 4
Calculated Molecular Weight 385 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC074782
Gene Symbol F2RL3
Gene ID (NCBI) 9002
RRIDAB_2880022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RI0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coagulation factor II receptor-like 3 (F2RL3) encodes a member of the protease-activated receptor subfamily, also known as protease-activated receptor 4 (PAR4), which takes partin platelet activation, intimal hyperplasia and inflammation (PMID:34284820). An absence of PAR4 in mouse models results in impaired hemostasis and a protection against pulmonary embolism, and a small number of missense coding variants in F2RL3 that alter platelet aggregation and function have been described(PMID:35012325). The calculated molecular weight and observed molecular weight of F2RL3 are both 41 kDa.

实验方案

Product Specific Protocols
WB protocol for F2RL3 antibody 25306-1-APDownload protocol
IHC protocol for F2RL3 antibody 25306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice.

Authors - BuChun Zhang
View Article
mouseWB

Front Oncol

Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity.

Authors - Nathalia Araujo
View Article
mouseWB

Neurosci Bull

Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.

Authors - Jianing Luo
View Article