验证数据展示
经过测试的应用
| Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, RAW 264.7 cells |
| Positive IF-Fro detected in | mouse spleen tissue |
| Positive IF/ICC detected in | RAW 264.7 cells |
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2000-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:750-1:3000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
81668-1-RR targets F4/80 in IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse |
| 免疫原 | F4/80 fusion protein Ag28252 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
| 别名 | Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1 |
| 计算分子量 | 102 kDa |
| GenBank蛋白编号 | NM_010130 |
| 基因名称 | F4/80 |
| Gene ID (NCBI) | 13733 |
| RRID | AB_2935571 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q61549 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for F4/80 antibody 81668-1-RR | Download protocol |
| IF protocol for F4/80 antibody 81668-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Integrin alpha1 beta1 promotes interstitial fibrosis in a mouse model of polycystic kidney disease | ||
Cell Rep The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring |
